Overview

  • Product nameRabbit Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesSheep
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Sheep IgG whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes

    Horseradish Peroxidase (HRP).

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/4000. PubMed: 15319485
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
IHC-Fr Use at an assay dependent concentration.

Rabbit Anti-Sheep IgG H&L (HRP) images

  • Ab6747 was used at dilution 1/2000 with the primary antibody ab7832 in WB. See the review on ab7832.
  • Ab6747 was used at dilution 1/100 with the primary antibody ab53842 in IHC-P. See the review on ab53842.

References for Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

This product has been referenced in:
  • Kälble F  et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016). IHC-P . Read more (PubMed: 27755542) »
  • Risitano R  et al. Flavonoid fraction of Bergamot juice reduces LPS-induced inflammatory response through SIRT1-mediated NF-?B inhibition in THP-1 monocytes. PLoS One 9:e107431 (2014). Read more (PubMed: 25260046) »

See all 13 Publications for this product

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Please find attached the Certificates of Compliance for ab6747 and ab53586.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"