Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed (ab7111)
Key features and details
- Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Rabbit
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
Overview
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Product name
Rabbit Anti-Sheep IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Rabbit -
Target species
Sheep -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
Minimal
cross-reactivity
Human more details -
Immunogen
Full length native Sheep IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Horseradish Peroxidase (HRP) -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/300 - 1/1500.
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IHC-P |
Use at an assay dependent dilution.
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|
WB |
1/500 - 1/3000.
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|
ELISA |
1/5000 - 1/22000.
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|
Immunomicroscopy |
Use at an assay dependent dilution.
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|
Dot blot |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/300 - 1/1500. |
IHC-P
Use at an assay dependent dilution. |
WB
1/500 - 1/3000. |
ELISA
1/5000 - 1/22000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Dot blot
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab7111 has been referenced in 5 publications.
- Cooper SA et al. Pathologic light chain amyloidosis oligomer detection in urinary extracellular vesicles as a diagnostic tool for response and progression of disease. Front Oncol 12:978198 (2022). PubMed: 36267963
- Legscha KJ et al. ?133p53a enhances metabolic and cellular fitness of TCR-engineered T cells and promotes superior antitumor immunity. J Immunother Cancer 9:N/A (2021). PubMed: 34112738
- Dinamarca MC et al. Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing. Nat Commun 10:1331 (2019). PubMed: 30902970
- Schmidt N et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). PubMed: 29056295
- Kristiansson MH et al. Comparative time-dependent analysis of potential inflammation biomarkers in lymphoma-bearing SJL mice. J Proteome Res 6:1735-44 (2007). PubMed: 17388619