Rabbit Anti-Sheep IgM H&L (Alkaline Phosphatase) (ab112761)
Key features and details
- Rabbit Anti-Sheep IgM H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-P, ICC/IF
Related conjugates and formulations
Overview
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Product name
Rabbit Anti-Sheep IgM H&L (Alkaline Phosphatase)
See all IgM secondary antibodies -
Host species
Rabbit -
Target species
Sheep -
Specificity
By immunoelectrophoresis and ELISA ab112761 reacts specifically with sheep IgM. May cross react with IgM from other species. -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Immunogen
Other Immunogen Type corresponding to IgM.
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.01% Magnesium chloride, 0.001% Zinc chloride, 1.19% HEPES -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. ab112761 was isolated by affinity chromatography using antigen coupled to agarose beads. -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. for chemiluminescent detection
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ELISA |
1/1000 - 1/10000. as a primary
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. for chemiluminescent detection |
ELISA
1/1000 - 1/10000. as a primary |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab112761 has been referenced in 1 publication.
- Rodriguez M et al. Transplanting FVIII/ET3-secreting cells in fetal sheep increases FVIII levels long-term without inducing immunity or toxicity. Nat Commun 14:4206 (2023). PubMed: 37452013