Rabbit specific HRP/AEC (ABC) Detection IHC Kit (ab64260)

Overview

  • Product nameRabbit specific HRP/AEC (ABC) Detection IHC Kit
    See all HRP/AEC Detection Kit kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • Notes

    Contains hydrogen peroxide and Sodium azide.

  • Tested applicationsIHC-Pmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 15 ml
    AEC Single Solution 1 x 15ml
    Biotinylated goat anti-rabbit IgG(H+L) 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research Areas
  • RelevanceAn HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • Alternative names
    • carbazole
    • Horseradish Peroxidase

Applications

Our Abpromise guarantee covers the use of ab64260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Rabbit specific HRP/AEC (ABC) Detection IHC Kit images

  • Human tonsil stained with anti-CD3 antibody using Anti Rabbit HRP/AEC Detection Kit (ab64260)

References for Rabbit specific HRP/AEC (ABC) Detection IHC Kit (ab64260)

ab64260 has not yet been referenced specifically in any publications.

Product Wall



Our ab93705 (Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit) is not suitable for use on mouse samples. The secondary antibody is a cocktail of biotinylated anti-mouse and anti-rabbit antibodies and the anti-mouse antibodies will re...

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Thank you for replying with that information.

As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)f...

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Thank you for contacting us. I can confirm the protein block has the following composition: PBS solution, pH 7.6 0.5% BSA 0.5% casein Less than 0.1% sodium azide I hope this information helps. Please do not hesitate to contact me shou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"