Overview

  • Product name
    Rabbit specific IHC polymer detection kit HRP/DAB
  • Product overview

    Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy. Signal amplification is achieved using an indirect polymer detection system. As Rabbit specific IHC polymer detection kit HRP/DAB does not use avidin or biotin, nonspecific staining from endogenous avidin-biotin activity is eliminated and there is no need for additional steps to block endogenous biotin. The primary antibody specific to an antigen on the tissue section is detected by primary antibody Amplifier. The HRP polymer is added to enable detection with signal amplification. Finally, antibody-bound antigen sites are visualized by adding an appropriate substrate/chromogen (not provided).

  • Tested applications
    Suitable for: IHCmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    Amplifier 1 x 15ml
    Detector 1 x 15ml

    Applications

    Our Abpromise guarantee covers the use of ab209101 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC Use at an assay dependent concentration.

    Rabbit specific IHC polymer detection kit HRP/DAB images

    • Immunohistochemistry of human melanoma tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemistry of human tonsil tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Protocols

    References for Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

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