RABEP1 is a Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A.
All lanes : Anti-RABEP1 antibody (ab176578) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg Lane 2 : HeLa whole cell lysates at 15 µg Lane 3 : HeLa whole cell lysates at 5 µg Lane 4 : 293T whole cell lysates at 50 µg Lane 5 : NIH3T3 whole cell lysates at 50 µg
Detection of RABEP1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176578 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176578 antibody which recognizes an upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 3 seconds.