Overview

  • Product name
  • Description
    Rabbit polyclonal to RABEP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human RABEP1 aa 812-862 (C terminal). The exact sequence is proprietary. NP_004694.2
    Sequence:

    DVSEQVQRD FVKLSQTLQV QLERIRQADS LERIRAILND TKLTDINQLP ET


    Database link: Q15276

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    RABEP1 is a Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A.
  • Cellular localization
    Cytoplasm. Early endosome. Recycling endosome
  • Database links
  • Alternative names
    • Neurocrescin antibody
    • Rab GTPase binding effector protein 1 antibody
    • RAB5EP antibody
    • Rabaptin 4 antibody
    • Rabaptin 5 antibody
    • Rabaptin 5alpha antibody
    • RABPT5 antibody
    • RABPT5A antibody
    • Renal carcinoma antigen NY REN 17 antibody
    • Renal carcinoma antigen NYREN17 antibody
    see all

Images

  • All lanes : Anti-RABEP1 antibody (ab176578) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg
    Lane 5 : NIH3T3 whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 99 kDa


    Exposure time : 30 seconds
  • Detection of RABEP1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176578 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176578 antibody which recognizes an upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab176578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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