Overview

  • Product name
  • Description
    Rabbit polyclonal to Rabex5
  • Tested applications
    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Tilapia, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 475-525 of Human Rabex5 (BAC87138.1).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
    • Sequence similarities
      Contains 1 A20-type zinc finger.
      Contains 1 VPS9 domain.
    • Post-translational
      modifications
      Monoubiquitinated.
    • Cellular localization
      Cytoplasm. Early endosome. Recycling endosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • RAB guanine nucleotide exchange factor (GEF) 1 antibody
      • Rab5 GDP/GTP exchange factor antibody
      • Rab5ef antibody
      • Rabaptin 5 associated exchange factor for Rab5 antibody
      • Rabaptin-5-associated exchange factor for Rab5 antibody
      • Rabex 5 antibody
      • Rabex-5 antibody
      • RABGEF 1 antibody
      • Rabgef1 antibody
      • RABX 5 antibody
      • RABX5 antibody
      • RABX5_HUMAN antibody
      • RAP1 antibody
      • Rin 2 antibody
      • Rin2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Rabex5 with ab103520 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Rabex5 by Western Blot of Immunprecipitate.
      An antibody to Rabex5 at 1µg/ml staining Rabex5 in HeLa whole cell lysate immunoprecipitated using ab103520 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab103520 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab103520.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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