The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notesIs unsuitable for WB.
FunctionRab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Rabex5 with ab103520 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Rabex5 antibody (ab103520)
Detection of Rabex5 by Western Blot of Immunprecipitate.
An antibody to Rabex5 at 1µg/ml staining Rabex5 in HeLa whole cell lysate immunoprecipitated using ab103520 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-Rabex5 antibody (ab103520)
has not yet been referenced specifically in any publications.
Publishing research using ab103520? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"