The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for WB.
Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Rabex5 with ab103520 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Rabex5 antibody (ab103520)
Detection of Rabex5 by Western Blot of Immunprecipitate.
An antibody to Rabex5 at 1µg/ml staining Rabex5 in HeLa whole cell lysate immunoprecipitated using ab103520 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.