Overview

  • Product name
    Anti-RABGAP1 antibody - C-terminal
  • Description
    Rabbit polyclonal to RABGAP1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within C terminal amino acids 720-1058 of Human RABGAP1.

  • Positive control
    • NT2D1, IMR32, U-87 MG, MCF7 and Mouse brain lysates; HeLa cells; BT474 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab153992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 121 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.
  • Cellular localization
    Cytoplasm, cytosol. Centrosome
  • Database links
  • Alternative names
    • GAP and centrosome associated protein antibody
    • GAPCENA antibody
    • HSPC094 antibody
    • Rab GTPase activating protein 1 antibody
    • Rab6 GTPase activating protein GAPCenA antibody
    • RP11-123N4.2 antibody
    • TBC1 domain family member 11 antibody
    • TBC1D11 antibody
    see all

Images

  • Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/10000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 121 kDa



    7.5% SDS PAGE
  • Anti-RABGAP1 antibody - C-terminal (ab153992) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 121 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling RABGAP1 with ab153992 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling RABGAP1 with ab153992 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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