The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 65 kDa.Can be blocked with RABGGTA peptide (ab178800). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionCatalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.
Sequence similaritiesBelongs to the protein prenyltransferase subunit alpha family. Contains 5 LRR (leucine-rich) repeats. Contains 6 PFTA repeats.