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Rabbit polyclonal to Rabphilin 3A
ab59259 detects endogenous levels of total Rabphilin 3A protein.
Predicted to work with:
Synthetic non-phosphopeptide derived from human Rabphilin 3A around the phosphorylation site of serine 234 (R-A-S
IHC-P: human brain tissue
WB: HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins)
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab59259 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Contains 2 C2 domains.
Contains 1 FYVE-type zinc finger. Contains 1 RabBD (Rab-binding) domain.
Cell junction > synapse.
Information by UniProt
Exophilin 1 antibody
Western blot - Rabphilin 3A antibody (ab59259)
All lanes : Anti-Rabphilin 3A antibody (ab59259) at 1/500 dilution Lane 1 : HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins) Lane 2 : HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins) with immunising peptide Predicted band size: 77 kDa Observed band size: 77 kDa
Immunohistochemistry (Paraffin-embedded sections) - Rabphilin 3A antibody (ab59259)
ab59259 at 1/50 dilution staining Rabphilin 3A in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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