Overview

  • Product nameAnti-Rabphilin 3A antibody
    See all Rabphilin 3A primary antibodies
  • Description
    Rabbit polyclonal to Rabphilin 3A
  • Specificityab59259 detects endogenous levels of total Rabphilin 3A protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Rabphilin 3A around the phosphorylation site of serine 234 (R-A-SP-E-A).

  • Positive control
    • IHC-P: human brain tissue WB: HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab59259 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

Target

Anti-Rabphilin 3A antibody images

  • All lanes : Anti-Rabphilin 3A antibody (ab59259) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins)
    Lane 2 : HeLa cell extracts treated with TNF alpha (20ng/ml, 2mins) with immunising peptide


    Predicted band size : 77 kDa
    Observed band size : 77 kDa
  • ab59259 at 1/50 dilution staining Rabphilin 3A in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Rabphilin 3A antibody (ab59259)

ab59259 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"