- Datasheet
- Specific References (24)
- Protocols
Overview
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Product nameAnti-Rac1 antibody [23A8]
See all Rac1 primary antibodies -
DescriptionMouse monoclonal [23A8] to Rac1
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SpecificityRecognizes Rac at 21kD.Slight Rac 2 cross-reactivity. The clone number has been updated from (0.T.127) to (23A8) both clone numbers name the same antibody clone.
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Tested applicationsSuitable for: IHC-Fr, WB, Flow Cyt, IHC (PFA fixed), ICC/IF, ELISA, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human
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Immunogen
Recombinant full length protein corresponding to Human Rac1.
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Positive control
- WB/IP: rat brain microsomal protein preparation, Mouse brain IHC-P: Mouse intestine tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 40% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number23A8
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IsotypeIgG2b
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab33186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.3 - 2 µg/ml. Predicted molecular weight: 21 kDa. | |
| Flow Cyt | Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC (PFA fixed) | 1/10 - 1/50. | |
| ICC/IF | 1/10 - 1/50. | |
| ELISA | 1/100 - 1/1000. | |
| IP | Use 1µg for 107 cells. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionPlasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. -
Tissue specificityIsoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
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Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
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DomainThe effector region mediates interaction with DEF6.
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Post-translational
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
Cellular localizationCell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 5879 Human
- Entrez Gene: 19353 Mouse
- Entrez Gene: 363875 Rat
- Omim: 602048 Human
- SwissProt: P63000 Human
- SwissProt: P63001 Mouse
- SwissProt: Q6RUV5 Rat
- Unigene: 413812 Human
see all -
FormRac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
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Alternative names
- Cell migration inducing gene 5 protein antibody
- Cell migration-inducing gene 5 protein antibody
- MGC111543 antibody
see all
Anti-Rac1 antibody [23A8] images
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![Western blot - Anti-Rac1 antibody [23A8] (ab33186)](http://a.abcam.com/ps/products/33/ab33186/Images/ab33186-269880-ab33186WB.jpg)
Western blot - Anti-Rac1 antibody [23A8] (ab33186)Anti-Rac1 antibody [23A8] (ab33186) at 0.3 µg/ml + Rat Brain Microsomes at 40 µg
Predicted band size : 21 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rac1 antibody [23A8] (ab33186)](http://a.abcam.com/ps/products/33/ab33186/Images/Rac1-Primary-antibodies-ab33186-16.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rac1 antibody [23A8] (ab33186)This image is courtesy of an anonymous Abreviewab33186 staining Rac1 in Mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA 1 hour at 20°C ; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. An HRP-conjugated Goat anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody. -
![Western blot - Anti-Rac1 antibody [23A8] (ab33186)](http://a.abcam.com/ps/products/33/ab33186/Images/Rac1-Primary-antibodies-ab33186-14.jpg)
Western blot - Anti-Rac1 antibody [23A8] (ab33186)This image is courtesy of an anonymous AbreviewAnti-Rac1 antibody [23A8] (ab33186) at 1/750 dilution (for 14 hours at 4°C) + Mouse brain tissue lysate at 15 µg
Secondary
An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 21 kDa
Observed band size : 21 kDa
Exposure time : 2 minutesThis image is courtesy of an anonymous Abreview
Blocking Step: 5% Milk for 1 hour at 22°C
Protocols
References for Anti-Rac1 antibody [23A8] (ab33186)
This product has been referenced in:
- Zhai L et al. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS One 12:e0174414 (2017). WB ; Rat . Read more (PubMed: 28388666) »
- Zhao W et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep 15:3048-3054 (2017). WB ; Human . Read more (PubMed: 28358422) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"