Overview

  • Product nameAnti-Rac1 antibody
    See all Rac1 primary antibodies
  • Description
    Rabbit polyclonal to Rac1
  • SpecificityRecognizes Rac1b specifically. Does not recognize other Rac1 isoforms.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the internal region of Human Rac1b (the immunogen sequence is not present in the other Rac1 isoforms)

  • Positive control
    • WB: HeLa and MCF7 cell lysates. IHC=P: Human colorectal, prostate or squamous cells carcinoma. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab78139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/50.
    IHC-P: 1/1000. Antigen retrieval using citrate pH6.0 is recommended.
    WB: 1/1000. Detects a band of approximately 22 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
      Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
    • Tissue specificityIsoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
    • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
    • DomainThe effector region mediates interaction with DEF6.
    • Post-translational
      modifications
      AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    • Cellular localizationCell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • FormRac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
    • Alternative names
      • Cell migration inducing gene 5 protein antibody
      • Cell migration-inducing gene 5 protein antibody
      • MGC111543 antibody
      • MIG5 antibody
      • Migration inducing gene 5 antibody
      • Migration inducing protein 5 antibody
      • p21 Rac1 antibody
      • p21-Rac1 antibody
      • Rac 1 antibody
      • RAC1 antibody
      • RAC1_HUMAN antibody
      • Ras like protein TC25 antibody
      • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody
      • Ras-like protein TC25 antibody
      • Ras-related C3 botulinum toxin substrate 1 antibody
      • Rho family small GTP binding protein Rac1 antibody
      • TC 25 antibody
      • TC25 antibody
      see all

    Anti-Rac1 antibody images

    • All lanes : Anti-Rac1 antibody (ab78139) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate

      Secondary
      Goat anti-Rabbit HRP-conjugated at 1/5000 dilution

      Observed band size : 22 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
    • Immunofluorescence analysis of HeLa cells using ab78139 at 1/50 dilution (yellow) in conjunction with a Donkey anti-Rabbit IgG Cy3 conjugated. Hela cells were fixed with 3.7% paraformaldehyde and permeabilized with a non-ionic detergent. The cells are dual stained with DAPI (blue).
    • Immunohistochemistry analysis of paraffin-embedded Human tubular colorectal carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining.
    • Immunohistochemistry analysis of paraffin-embedded Human prostate carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive plasma membrane staining with some stromal background staining.
    • Immunohistochemistry analysis of paraffin-embedded Human squamous cells carcinoma using ab78139 at 1/1000 dilution with an HRP-DAB detection system. Tissue pretreated with citrate pH6.0 antigen retrieval. Immunoreactivity is seen primarily as diffusive nuclear staining pattern.

    References for Anti-Rac1 antibody (ab78139)

    ab78139 has not yet been referenced specifically in any publications.

    Product Wall

    The correct storage buffer of antibody ab78139 is as follows; 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, 0.05% Sodium Azide and 30% Glycerol. It does contain Glycerine.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"