Overview

  • Product name
  • Description
    Rabbit polyclonal to Rac1
  • Specificity
    ab97732 detects endogenous levels of total Rac1 protein.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non phosphopeptide derived from around amino acids 69-73 of Human Rac1 (

    PLSYP

    ).

  • Positive control
    • HeLa cells; A431 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab97732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
    Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
  • Tissue specificity
    Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rho family.
  • Domain
    The effector region mediates interaction with DEF6.
  • Post-translational
    modifications
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization
    Cell membrane. Melanosome. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Form
    Rac1 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
  • Alternative names
    • Cell migration inducing gene 5 protein antibody
    • Cell migration-inducing gene 5 protein antibody
    • MGC111543 antibody
    • MIG5 antibody
    • Migration inducing gene 5 antibody
    • Migration inducing protein 5 antibody
    • p21 Rac1 antibody
    • p21-Rac1 antibody
    • Rac 1 antibody
    • RAC1 antibody
    • RAC1_HUMAN antibody
    • Ras like protein TC25 antibody
    • Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody
    • Ras-like protein TC25 antibody
    • Ras-related C3 botulinum toxin substrate 1 antibody
    • Rho family small GTP binding protein Rac1 antibody
    • TC 25 antibody
    • TC25 antibody
    see all

Images

  • All lanes : Anti-Rac1 antibody (ab97732) at 1/500 dilution

    Lane 1 : A431 cell extracts with immunizing peptide
    Lane 2 : A431 cell extracts,


    Predicted band size : 21 kDa
  • ab97732 at 1/100 dilution staining Rac1 in methanol-fixed HeLa cells by Immunofluorescence.

References

This product has been referenced in:
  • Feng H  et al. Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation. Nanoscale Res Lett 11:240 (2016). ICC/IF ; Rat . Read more (PubMed: 27142878) »
  • Montagner A  et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF ; Mouse . Read more (PubMed: 24203162) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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