Recombinant Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y]
See all RAC1 + Cdc42 primary antibodies -
Description
Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cells treated with Lambda Phosphatase.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP221Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 23 kDa.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 23 kDa. |
Target
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Relevance
Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK. -
Cellular localization
Cell membrane; Lipid anchor; Cytoplasmic side. -
Database links
- Entrez Gene: 5879 Human
- Entrez Gene: 998 Human
- Omim: 116952 Human
- Omim: 602048 Human
- SwissProt: P60953 Human
- SwissProt: P63000 Human
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Alternative names
- CDC42Hs antibody
- Cell division cycle 42 antibody
- G25K antibody
see all
Images
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All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilution
Lane 1 : A431 cell lysate treated with Lambda Phosphatase
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 24 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76535 has been referenced in 1 publication.
- Liu Y et al. Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway. Genes Dis 8:232-240 (2021). PubMed: 33997170