Recombinant
RabMAb

Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)

Overview

  • Product name
    Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y]
    See all RAC1 + Cdc42 primary antibodies
  • Description
    Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71)
  • Specificity
    This antibody only detects RAC1 + Cdc42 phosphorylated on Serine 71.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho peptide corresponding to residues surrounding Serine 71 of human RAC1 + Cdc42

  • Positive control
    • WB: A431 cells treated with Lambda Phosphatase.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
    • Cellular localization
      Cell membrane; Lipid anchor; Cytoplasmic side.
    • Database links
    • Alternative names
      • CDC42Hs antibody
      • Cell division cycle 42 antibody
      • G25K antibody
      • MIG5 antibody
      • Migration inducing gene 5 antibody
      • p21 Rac1 antibody
      • Ras like protein TC25 antibody
      • Small GTP binding protein Cdc42 antibody
      • TC25 antibody
      see all

    Images

    • All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilution

      Lane 1 : A431 cell lysate treated with Lambda Phosphatase
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    References

    ab76535 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76535.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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