Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)

Overview

  • Product nameAnti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y]
    See all RAC1 + Cdc42 primary antibodies
  • Description
    Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71)
  • SpecificityThis antibody only detects RAC1 + Cdc42 phosphorylated on Serine 71.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho peptide corresponding to residues surrounding Serine 71 of human RAC1 + Cdc42

  • Positive control
    • WB: A431 cells treated with Lambda Phosphatase.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • RelevanceCdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
    • Cellular localizationCell membrane; Lipid anchor; Cytoplasmic side.
    • Database links
    • Alternative names
      • CDC42Hs antibody
      • Cell division cycle 42 antibody
      • G25K antibody
      • MIG5 antibody
      • Migration inducing gene 5 antibody
      • p21 Rac1 antibody
      • Ras like protein TC25 antibody
      • Small GTP binding protein Cdc42 antibody
      • TC25 antibody
      see all

    Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] images

    • All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilution

      Lane 1 : A431 cell lysate treated with Lambda Phosphatase
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    References for Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)

    ab76535 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"