Overview

  • Product name
    Anti-RAC3 antibody [EPR6679(B)]
    See all RAC3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6679(B)] to RAC3
  • Specificity
    ab124943 cross-reacts with Rac1, Rac2 and Cdc42
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human RAC3

  • Positive control
    • Human heart tissue, Human placenta tissue; JAR cells; Human fetal brain lysate, SH-SY5Y, JAR and BxPC-3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/50 - 1/100. antigen retrieval is recommeded.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Promotes cell adhesion and spreading on fibrinogen in a CIB1 and alpha-IIb/beta3 integrin-mediated manner.
    • Tissue specificity
      Highest levels in brain, also detected in heart, placenta and pancreas.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Rho family.
    • Post-translational
      modifications
      (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.
    • Cellular localization
      Cytoplasm. Endomembrane system. Cell projection, lamellipodium. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton. Membrane-associated when activated. Colocalizes with NRBP to endomembranes and at the cell periphery in lamellipodia. Colocalized with CIB1 in the perinuclear area and at the cell periphery.
    • Information by UniProt
    • Database links
    • Form
      Rac3 has high sequence homology with other Rac proteins. Therefore some antibodies may show cross-reactivity.
    • Alternative names
      • OTTMUSP00000004488 antibody
      • p21 Rac3 antibody
      • p21-Rac3 antibody
      • Rac1B antibody
      • RAC3 antibody
      • RAC3_HUMAN antibody
      • RAS related C3 botulinum substrate 3 antibody
      • Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody
      • Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) antibody
      • Ras-related C3 botulinum toxin substrate 3 antibody
      • Rho family small GTP binding protein Rac3 antibody
      • RP23-84C12.18 antibody
      see all

    Images

    • All lanes : Anti-RAC3 antibody [EPR6679(B)] (ab124943) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 21 kDa
    • ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human heart tissue by immunohistochemistry
    • ab124943, at a 1/50 dilution, staining RAC3 in paraffin-embedded Human placenta tissue by immunohistochemistry
    • ab124943, at a 1/50 dilution, staining RAC3 in JAR cells by immunofluorescence
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Gaitanos TN  et al. Tiam-Rac signaling mediates trans-endocytosis of ephrin receptor EphB2 and is important for cell repulsion. J Cell Biol 214:735-52 (2016). Read more (PubMed: 27597758) »
    • Wang Y  et al. Inhibition of prostate smooth muscle contraction and prostate stromal cell growth by the inhibitors of Rac, NSC23766 and EHT1864. Br J Pharmacol 172:2905-17 (2015). Read more (PubMed: 25631101) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124943.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up