Overview

  • Product name
  • Description
    Goat polyclonal to RACGAP1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: GRQGNFFASPMLK, corresponding to C terminal amino acids 620-632 of Human RACGAP1.

  • Positive control
    • Human testis lysate. K562 cells.
  • General notes
    Principal Names - RACGAP1; Rac GTPase activating protein 1; ID-GAP; MgcRacGAP; GTPase activating protein. Official Gene Symbol - - RACGAP1. GenBank Accession Number – NP_037409. LocusLink ID - 29127.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).Can be blocked with Human RACGAP1 peptide (ab22898).
IP Use at an assay dependent dilution.

Target

  • Function
    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • Tissue specificity
    Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
  • Sequence similarities
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Rho-GAP domain.
  • Domain
    The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • Post-translational
    modifications
    Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYK4 antibody
    • GAP antibody
    • Gap1 antibody
    • GTPase activating protein antibody
    • HsCYK-4 antibody
    • ID GAP antibody
    • KIAA1478 antibody
    • Male germ cell RacGap antibody
    • MgcRacGAP antibody
    • Protein CYK4 homolg antibody
    • Protein CYK4 homolog antibody
    • Rac GTPase activating protein 1 antibody
    • Rac GTPase-activating protein 1 antibody
    • RACGAP 1 antibody
    • Racgap1 antibody
    • RGAP1_HUMAN antibody
    see all

Anti-RACGAP1 antibody images

  • Lane 1 :
    Lane 2 :

    Lane 1 : K562 cell lysate (in RIPA buffer)
    Lane 2 : Jurkat cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 71 kDa

    Primary incubation was 1 hour. Detected by chemiluminescence.

     

  • ab2270 (1µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer (pH9), HRP-staining.
  • Anti-RACGAP1 antibody (ab2270) at 0.2 µg/ml + Human Testis lysate (RIPA buffer) at 30 µg

    Predicted band size : 71 kDa
    ab2270 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Anti-RACGAP1 antibody (ab2270) at 1 µg/ml + K562 cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 71 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-RACGAP1 antibody (ab2270)

This product has been referenced in:

See all 11 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cell (HT1080)
Specification
HT1080
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde
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Verified customer

Submitted Jan 21 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (HT1080)
Specification
HT1080
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jan 13 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
Username

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Verified customer

Submitted Dec 16 2008

Thank you for your e-mail. The lysate used was Human Testis lysate lysed in RIPA buffer, the recipe for that buffer is as detailed below: 50mM Tris HCl pH 8 150 mM NaCl 1% NP-40 0.5% sodium Deoxycholate 0.1% SDS protease inhibitor cocktail (...

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Thank you for your enquiry. This antibody has been tested on Human Testis lysate, therefore we would strongly suggest using this type of lysate as positive control along with the samples. Should your customer still have difficulty with this pro...

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