Overview

  • Product nameAnti-RACGAP1 antibody
    See all RACGAP1 primary antibodies
  • Description
    Mouse monoclonal to RACGAP1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-110 of Human RACGAP1

Properties

Applications

Our Abpromise guarantee covers the use of ab57081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 1 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
    • Tissue specificityHighly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
    • Sequence similaritiesContains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Rho-GAP domain.
    • DomainThe coiled coil region is indispensible for localization to the midbody during cytokinesis.
    • Post-translational
      modifications
      Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
    • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
    • Information by UniProt
    • Database links
    • Alternative names
      • CYK4 antibody
      • GAP antibody
      • Gap1 antibody
      • GTPase activating protein antibody
      • HsCYK-4 antibody
      • ID GAP antibody
      • KIAA1478 antibody
      • Male germ cell RacGap antibody
      • MgcRacGAP antibody
      • Protein CYK4 homolg antibody
      • Protein CYK4 homolog antibody
      • Rac GTPase activating protein 1 antibody
      • Rac GTPase-activating protein 1 antibody
      • RACGAP 1 antibody
      • Racgap1 antibody
      • RGAP1_HUMAN antibody
      see all

    Anti-RACGAP1 antibody images

    • RACGAP1 antibody (ab57081) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human testis.


    • Predicted band size : 71 kDa
      RACGAP1 antibody (ab57081) at 1ug/lane + K-562 cell lysate at 25ug/lane.

    References for Anti-RACGAP1 antibody (ab57081)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abreviews
    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions Reduced Denaturing (7.5%)
    Loading amount 30 µg
    Specification Kidney
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Nov 02 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"