Anti-RACGAP1 (phospho S387) antibody (ab61078)

Overview

  • Product name
    Anti-RACGAP1 (phospho S387) antibody
    See all RACGAP1 primary antibodies
  • Description
    Rabbit polyclonal to RACGAP1 (phospho S387)
  • Specificity
    ab61078 detects endogenous levels of RACGAP1 only when phosphorylated at serine 387.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human GTPase Activating Protein around the phosphorylation site of serine 387 (R-I-SP-G-C).

  • Positive control
    • COS7 cells extract HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 71 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • Tissue specificity
    Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
  • Sequence similarities
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Rho-GAP domain.
  • Domain
    The coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • Post-translational
    modifications
    Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CYK4 antibody
    • GAP antibody
    • Gap1 antibody
    • GTPase activating protein antibody
    • HsCYK-4 antibody
    • ID GAP antibody
    • KIAA1478 antibody
    • Male germ cell RacGap antibody
    • MgcRacGAP antibody
    • Protein CYK4 homolg antibody
    • Protein CYK4 homolog antibody
    • Rac GTPase activating protein 1 antibody
    • Rac GTPase-activating protein 1 antibody
    • RACGAP 1 antibody
    • Racgap1 antibody
    • RGAP1_HUMAN antibody
    see all

Anti-RACGAP1 (phospho S387) antibody images

  • All lanes : Anti-RACGAP1 (phospho S387) antibody (ab61078) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, minus immunising peptide
    Lane 2 : Extracts from COS7 cells, plus immunising peptide


    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells, using ab61078 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

References for Anti-RACGAP1 (phospho S387) antibody (ab61078)

ab61078 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Treatment
Nocodazole synchronization for overnight, and then Cdk inhibitor purvalanol A (20uM) for 20 minutes
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Mar 23 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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