Overview

  • Product name
  • Description
    Rabbit polyclonal to RACK1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human RACK1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P63244

  • Positive control
    • WB: Human and mouse liver tissue lysate and HeLa, MCF7, NIH3T3, Jurkat and HepG2 whole cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab111621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
  • Sequence similarities
    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Domain
    The WD repeats domain 5 mediates interaction with TRIM63.
  • Cellular localization
    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 21 protein antibody
    • GBLP_HUMAN antibody
    • Gnb2-rs1 antibody
    • Gnb2l1 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
    • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
    • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
    • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
    • H12.3 antibody
    • HLC-7 antibody
    • Human lung cancer oncogene 7 protein antibody
    • lung cancer oncogene 7 antibody
    • OTTHUMP00000223704 antibody
    • OTTHUMP00000223870 antibody
    • OTTHUMP00000223891 antibody
    • OTTHUMP00000223893 antibody
    • OTTHUMP00000223900 antibody
    • OTTHUMP00000223902 antibody
    • OTTHUMP00000223930 antibody
    • OTTHUMP00000223931 antibody
    • PIG21 antibody
    • Proliferation inducing gene 21 antibody
    • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
    • RACK1 antibody
    • Receptor for activated C kinase 1 antibody
    • Receptor for activated C kinase antibody
    • Receptor of activated protein kinase C 1 antibody
    see all

Images

  • ICC/IF image of ab111621 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111621, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.

  • All lanes : Anti-RACK1 antibody (ab111621) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 38 kDa (possible non-specific binding),54 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab111621 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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