The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).
FunctionSeems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similaritiesBelongs to the WD repeat G protein beta family. Contains 7 WD repeats.
DomainThe WD repeats domain 5 mediates interaction with TRIM63.
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.