Overview

  • Product name
  • Description
    Goat polyclonal to RACK1
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids 133-147 of Human RACK1 (NP_006089.1).

  • Positive control
    • Human Small Intestine tissue; Human Lymph Node lysate; Mouse and Rat Spleen lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab166796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
  • Sequence similarities
    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Domain
    The WD repeats domain 5 mediates interaction with TRIM63.
  • Cellular localization
    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 21 protein antibody
    • GBLP_HUMAN antibody
    • Gnb2-rs1 antibody
    • Gnb2l1 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
    • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
    • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
    • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
    • H12.3 antibody
    • HLC-7 antibody
    • Human lung cancer oncogene 7 protein antibody
    • lung cancer oncogene 7 antibody
    • OTTHUMP00000223704 antibody
    • OTTHUMP00000223870 antibody
    • OTTHUMP00000223891 antibody
    • OTTHUMP00000223893 antibody
    • OTTHUMP00000223900 antibody
    • OTTHUMP00000223902 antibody
    • OTTHUMP00000223930 antibody
    • OTTHUMP00000223931 antibody
    • PIG21 antibody
    • Proliferation inducing gene 21 antibody
    • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
    • RACK1 antibody
    • Receptor for activated C kinase 1 antibody
    • Receptor for activated C kinase antibody
    • Receptor of activated protein kinase C 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling RACK1 with ab166796 at 3.75 µg/ml.
  • Anti-RACK1 antibody (ab166796) at 0.1 µg/ml + Human Lymph Node lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 33 kDa (why is the actual band size different from the predicted?)



    Primary incubation: 1 hour.

References

ab166796 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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