Overview

  • Product name
  • Description
    Rabbit polyclonal to RACK1
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLWD LTTGTTTRRF conjugated to KLH, corresponding to N terminal amino acids 1-100 of Human RACK1

  • Positive control
    • Ramos cell lysate. Hepatocarcinoma formalin-fixed, paraffin embedded tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab37672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
  • Sequence similarities
    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Domain
    The WD repeats domain 5 mediates interaction with TRIM63.
  • Cellular localization
    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 21 protein antibody
    • GBLP_HUMAN antibody
    • Gnb2-rs1 antibody
    • Gnb2l1 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
    • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
    • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
    • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
    • H12.3 antibody
    • HLC-7 antibody
    • Human lung cancer oncogene 7 protein antibody
    • lung cancer oncogene 7 antibody
    • OTTHUMP00000223704 antibody
    • OTTHUMP00000223870 antibody
    • OTTHUMP00000223891 antibody
    • OTTHUMP00000223893 antibody
    • OTTHUMP00000223900 antibody
    • OTTHUMP00000223902 antibody
    • OTTHUMP00000223930 antibody
    • OTTHUMP00000223931 antibody
    • PIG21 antibody
    • Proliferation inducing gene 21 antibody
    • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
    • RACK1 antibody
    • Receptor for activated C kinase 1 antibody
    • Receptor for activated C kinase antibody
    • Receptor of activated protein kinase C 1 antibody
    see all

Images

  • Anti-RACK1 antibody (ab37672) at 1/100 dilution + Ramos cell lysate.

    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab37672, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab37672 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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