• Product name
  • Description
    Rabbit polyclonal to RACK1
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:


    , conjugated to KLH by a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-16 of Human RACK1

  • Positive control
    • Paraformaldehyde-fixed HeLa cells, HEK-293T cell lysate.



Our Abpromise guarantee covers the use of ab62735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
IP Use at an assay dependent dilution. Use 5-10 µL.
ICC/IF 1/100 - 1/200.


  • Function
    Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
  • Sequence similarities
    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Domain
    The WD repeats domain 5 mediates interaction with TRIM63.
  • Cellular localization
    Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 21 protein antibody
    • GBLP_HUMAN antibody
    • Gnb2-rs1 antibody
    • Gnb2l1 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
    • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
    • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
    • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
    • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
    • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
    • H12.3 antibody
    • HLC-7 antibody
    • Human lung cancer oncogene 7 protein antibody
    • lung cancer oncogene 7 antibody
    • OTTHUMP00000223704 antibody
    • OTTHUMP00000223870 antibody
    • OTTHUMP00000223891 antibody
    • OTTHUMP00000223893 antibody
    • OTTHUMP00000223900 antibody
    • OTTHUMP00000223902 antibody
    • OTTHUMP00000223930 antibody
    • OTTHUMP00000223931 antibody
    • PIG21 antibody
    • Proliferation inducing gene 21 antibody
    • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
    • RACK1 antibody
    • Receptor for activated C kinase 1 antibody
    • Receptor for activated C kinase antibody
    • Receptor of activated protein kinase C 1 antibody
    see all


  • All lanes : Anti-RACK1 antibody (ab62735) at 1/500 dilution

    Lane 1 : Extract from hek-293T cell line
    Lane 2 : Extract from HeLa cell line
    Lane 3 : Extract from MCF7 cell line
    Lane 4 : Extract from U2OS cell line
    Lane 5 : Extract from Jurkat cell line
    Lane 6 : Extract from RAT1 cell line
    Lane 7 : Extract from PC12 cell line
    Lane 8 : Extract from NIH3T3 cell line

    All lanes : Goat Anti-Rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Yan Y & Jiang Y RACK1 affects glioma cell growth and differentiation through the CNTN2-mediated RTK/Ras/MAPK pathway. Int J Mol Med 37:251-7 (2016). WB ; Human . Read more (PubMed: 26718491) »
  • Coppari E  et al. A nanotechnological, molecular-modeling, and immunological approach to study the interaction of the anti-tumorigenic peptide p28 with the p53 family of proteins. Int J Nanomedicine 9:1799-813 (2014). WB ; Human . Read more (PubMed: 24748790) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you very much for your interest in ab62735.

We would guarantee ab62735 to work in ICC/IF with mouse cells. We have this reference citing previous use for ICC/IF (though not in mouse primary neurons). http://www.ncbi.nlm.nih.gov/pubmed/2...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (BMM)
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Sep 13 2010


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