The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 5-10 µg.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
Staining of the RACK1 band is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSeems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similaritiesBelongs to the WD repeat G protein beta family. Contains 7 WD repeats.
DomainThe WD repeats domain 5 mediates interaction with TRIM63.
Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Cell proliferation-inducing gene 21 protein antibody
Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
Guanine nucleotide binding protein beta subunit 2 like 1 antibody
Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
Guanine nucleotide binding protein subunit beta 2 like 1 antibody
Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
Human lung cancer oncogene 7 protein antibody
lung cancer oncogene 7 antibody
Proliferation inducing gene 21 antibody
Protein homologous to chicken B complex protein guanine nucleotide binding antibody
Receptor for activated C kinase 1 antibody
Receptor for activated C kinase antibody
Receptor of activated protein kinase C 1 antibody
Anti-RACK1 antibody images
Western blot - RACK1 antibody (ab62736)
All lanes : Anti-RACK1 antibody (ab62736) at 1 µg/ml
Lane 1 : Extract from HEK-293T cell line Lane 2 : Extract from HeLa cell line Lane 3 : Extract from MCF7 cell line Lane 4 : Extract from U2OS cell line Lane 5 : Extract from Jurkat cell line Lane 6 : Extract from RAT1 cell line Lane 7 : Extract from PC12 cell line Lane 8 : Extract from NIH3T3 cell line
Secondary Goat Anti-Rabbit IgG Developed using the ECL technique