Overview

  • Product nameAnti-RACK1 antibody
    See all RACK1 primary antibodies
  • Description
    Rabbit polyclonal to RACK1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide:

    C-DEL KQEVISTSSK AE

    conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to near C-terminal amino acids 268-282 of Human RACK1.

  • Positive control
    • HEK-293T cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 5-10 µg.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
    Staining of the RACK1 band is specifically inhibited by the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSeems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
    • Sequence similaritiesBelongs to the WD repeat G protein beta family.
      Contains 7 WD repeats.
    • DomainThe WD repeats domain 5 mediates interaction with TRIM63.
    • Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 21 protein antibody
      • GBLP_HUMAN antibody
      • Gnb2-rs1 antibody
      • Gnb2l1 antibody
      • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
      • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
      • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
      • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
      • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
      • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
      • H12.3 antibody
      • HLC-7 antibody
      • Human lung cancer oncogene 7 protein antibody
      • lung cancer oncogene 7 antibody
      • OTTHUMP00000223704 antibody
      • OTTHUMP00000223870 antibody
      • OTTHUMP00000223891 antibody
      • OTTHUMP00000223893 antibody
      • OTTHUMP00000223900 antibody
      • OTTHUMP00000223902 antibody
      • OTTHUMP00000223930 antibody
      • OTTHUMP00000223931 antibody
      • PIG21 antibody
      • Proliferation inducing gene 21 antibody
      • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
      • RACK1 antibody
      • Receptor for activated C kinase 1 antibody
      • Receptor for activated C kinase antibody
      • Receptor of activated protein kinase C 1 antibody
      see all

    Anti-RACK1 antibody images

    • All lanes : Anti-RACK1 antibody (ab62736) at 1 µg/ml

      Lane 1 : Extract from HEK-293T cell line
      Lane 2 : Extract from HeLa cell line
      Lane 3 : Extract from MCF7 cell line
      Lane 4 : Extract from U2OS cell line
      Lane 5 : Extract from Jurkat cell line
      Lane 6 : Extract from RAT1 cell line
      Lane 7 : Extract from PC12 cell line
      Lane 8 : Extract from NIH3T3 cell line

      Secondary
      Goat Anti-Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 35 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References for Anti-RACK1 antibody (ab62736)

    ab62736 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"