Overview

  • Product name
  • Description
    Mouse polyclonal to Rad1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-282 of Human Rad1 (NP_002844).

  • Positive control
    • Rad1 transfected 293T cell line lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab168397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
  • Tissue specificity
    Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
  • Sequence similarities
    Belongs to the rad1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle checkpoint protein Hrad1 antibody
    • Cell cycle checkpoint protein Rad 1 A / B antibody
    • Cell cycle checkpoint protein RAD1 antibody
    • Checkpoint control protein HRAD1 antibody
    • Checkpoint control protein RAD1 antibody
    • DNA repair exonuclease antibody
    • DNA repair exonuclease rad1 antibody
    • DNA repair exonuclease rad1 homolog antibody
    • DNA repair exonuclease REC1 antibody
    • DNA repair protein RAD1 antibody
    • EC 3.1.11.2 antibody
    • Exonuclease homolog RAD1 antibody
    • GTP-binding protein RAD antibody
    • hRAD 1 antibody
    • hRAD1 antibody
    • MGC77779 antibody
    • RAD 1 antibody
    • RAD antibody
    • RAD1 antibody
    • RAD1 homolog (S. pombe) antibody
    • RAD1 homolog antibody
    • Rad1 like DNA damage checkpoint antibody
    • Rad1 like DNA damage checkpoint protein antibody
    • RAD1, S. pombe, homolog of antibody
    • Rad1-like DNA damage checkpoint protein antibody
    • RAD1_HUMAN antibody
    • Ras associated with diabetes antibody
    • REC 1 antibody
    • REC1 antibody
    • RRAD antibody
    see all

Images

  • All lanes : Anti-Rad1 antibody (ab168397) at 1 µg/ml

    Lane 1 : Rad1 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Predicted band size: 32 kDa

  • Immunofluorescent analysis of HeLa cells labeling Rad1 with ab168397 at 10 µg/ml.

References

ab168397 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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