Overview

  • Product name
  • Description
    Rabbit polyclonal to Rad1
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    PLLTQQIQDEDDQYSLVA

    conjugated to KLH via a C-terminal Cysteine residue linker, corresponding to N terminal amino acids 2 - 19 of Human Rad1

  • Positive control
    • HeLa cell lysates; Extracts of HL-60 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum protein
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes
    IP: 5-10 ul immunoprecipitates RAD1 from HeLa cell lysates.
    WB: 1/250 - 1/500. Detects a band of approximately 30 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
    • Tissue specificity
      Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
    • Sequence similarities
      Belongs to the rad1 family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell cycle checkpoint protein Hrad1 antibody
      • Cell cycle checkpoint protein Rad 1 A / B antibody
      • Cell cycle checkpoint protein RAD1 antibody
      • Checkpoint control protein HRAD1 antibody
      • Checkpoint control protein RAD1 antibody
      • DNA repair exonuclease antibody
      • DNA repair exonuclease rad1 antibody
      • DNA repair exonuclease rad1 homolog antibody
      • DNA repair exonuclease REC1 antibody
      • DNA repair protein RAD1 antibody
      • EC 3.1.11.2 antibody
      • Exonuclease homolog RAD1 antibody
      • GTP-binding protein RAD antibody
      • hRAD 1 antibody
      • hRAD1 antibody
      • MGC77779 antibody
      • RAD 1 antibody
      • RAD antibody
      • RAD1 antibody
      • RAD1 homolog (S. pombe) antibody
      • RAD1 homolog antibody
      • Rad1 like DNA damage checkpoint antibody
      • Rad1 like DNA damage checkpoint protein antibody
      • RAD1, S. pombe, homolog of antibody
      • Rad1-like DNA damage checkpoint protein antibody
      • RAD1_HUMAN antibody
      • Ras associated with diabetes antibody
      • REC 1 antibody
      • REC1 antibody
      • RRAD antibody
      see all

    References

    ab50310 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab50310.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up