The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
FunctionComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Tissue specificityExpressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lateral ventricle wall using ab 76830 at 3 µg/ml.
References for Anti-Rad1 antibody (ab76830)
This product has been referenced in:
Osorio-Zambrano WF & Davey S Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling. PLoS One10:e0144434 (2015).
Read more (PubMed: 26658951) »