Overview

  • Product nameAnti-Rad1 antibody
    See all Rad1 primary antibodies
  • Description
    Mouse monoclonal to Rad1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant tagged fragment: MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL , corresponding to amino acids 1-90 of Human Rad1.

  • Positive control
    • HeLa cell lysate. RAD1 transfected 293T cell line. Human lateral ventricle wall tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • FunctionComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
  • Tissue specificityExpressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
  • Sequence similaritiesBelongs to the rad1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell cycle checkpoint protein Hrad1 antibody
    • Cell cycle checkpoint protein Rad 1 A / B antibody
    • Cell cycle checkpoint protein RAD1 antibody
    • Checkpoint control protein HRAD1 antibody
    • Checkpoint control protein RAD1 antibody
    • DNA repair exonuclease antibody
    • DNA repair exonuclease rad1 antibody
    • DNA repair exonuclease rad1 homolog antibody
    • DNA repair exonuclease REC1 antibody
    • DNA repair protein RAD1 antibody
    • EC 3.1.11.2 antibody
    • Exonuclease homolog RAD1 antibody
    • GTP-binding protein RAD antibody
    • hRAD 1 antibody
    • hRAD1 antibody
    • MGC77779 antibody
    • RAD 1 antibody
    • RAD antibody
    • RAD1 antibody
    • RAD1 homolog (S. pombe) antibody
    • RAD1 homolog antibody
    • Rad1 like DNA damage checkpoint antibody
    • Rad1 like DNA damage checkpoint protein antibody
    • RAD1, S. pombe, homolog of antibody
    • Rad1-like DNA damage checkpoint protein antibody
    • RAD1_HUMAN antibody
    • Ras associated with diabetes antibody
    • REC 1 antibody
    • REC1 antibody
    • RRAD antibody
    see all

Anti-Rad1 antibody images

  • Anti-Rad1 antibody (ab76830) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Anti-Rad1 antibody (ab76830) at 5 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-Rad1 antibody (ab76830) at 5 µg/ml

    Lane 1 : Rad1 transfected 293T cell line
    Lane 2 : Non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human lateral ventricle wall using ab 76830 at 3 µg/ml.

References for Anti-Rad1 antibody (ab76830)

ab76830 has not yet been referenced specifically in any publications.

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