Overview

  • Product name
  • Description
    Rabbit polyclonal to Rad17
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Rad17.
    Database link: O75943

  • Positive control
    • SW480, BT474, K562, 22RV-1 and Raji cell extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab186837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 77 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function
    Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
  • Tissue specificity
    Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
  • Sequence similarities
    Belongs to the rad17/RAD24 family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
  • Cellular localization
    Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCYC antibody
    • Cell cycle checkpoint protein antibody
    • Cell cycle checkpoint protein RAD17 antibody
    • FLJ41520 antibody
    • HRAD 17 antibody
    • hRad17 antibody
    • R24L antibody
    • Rad 17 antibody
    • Rad 24 antibody
    • RAD1 (S. pombe) homolog antibody
    • RAD1 homolog antibody
    • RAD17 antibody
    • RAD17 homolog (S. pombe) antibody
    • RAD17 homolog antibody
    • Rad17 like protein antibody
    • RAD17, S. pombe, homolog of antibody
    • RAD17_HUMAN antibody
    • RAD17Sp antibody
    • Rad24 antibody
    • Rad24, mouse, homolog of antibody
    • Rad24, S. cerevisiae, homolog of antibody
    • RF C activator 1 homolog antibody
    • RF C/activator 1 homolog antibody
    • RF-C/activator 1 homolog antibody
    see all

Images

  • All lanes : Anti-Rad17 antibody (ab186837) at 1/500 dilution

    Lane 1 : SW480 cell extracts
    Lane 2 : BT474 cell extracts
    Lane 3 : K562 cell extracts
    Lane 4 : 22RV-1 cell extracts
    Lane 5 : Raji cell extracts


    Predicted band size : 77 kDa

References

ab186837 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186837.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up