Anti-Rad17 antibody - Carboxyterminal end (ab50526)


  • Product nameAnti-Rad17 antibody - Carboxyterminal end
    See all Rad17 primary antibodies
  • Description
    Rabbit polyclonal to Rad17 - Carboxyterminal end
  • SpecificityAb50526 specifically recognizes RAD17 (C terminal). The immunizing peptide is present in isoforms 1 to 4 of RAD17.
  • Tested applicationsSuitable for: IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: HSAEESLGEPTQATVPET, corresponding to amino acids 623-640 of Human Rad17 (isoform 2) conjugated to KLH by an N terminal Cysteine residue linker.

  • Positive control
    • HeLa cell lysates and HEK293-T cells fixed with methanol acetone.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAb50526 is affinity purified on the immunizing peptide immobilized on agarose.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab50526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: Use at a concentration of 5.0 - 10.0 µg/ml using HEK293-T cells fixed with methanol-acetone.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
    Staining of the RAD17 band is specifically inhibited by the immunizing peptide. It is recommended that the antibody be diluted in phosphate buffered saline containing 5% non-fat dry milk and 0.05 % TWEEN 20.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
    • Tissue specificityOverexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
    • Sequence similaritiesBelongs to the rad17/RAD24 family.
    • Post-translational
      Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
    • Cellular localizationNucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCYC antibody
      • Cell cycle checkpoint protein antibody
      • Cell cycle checkpoint protein RAD17 antibody
      • FLJ41520 antibody
      • HRAD 17 antibody
      • hRad17 antibody
      • R24L antibody
      • Rad 17 antibody
      • Rad 24 antibody
      • RAD1 (S. pombe) homolog antibody
      • RAD1 homolog antibody
      • RAD17 antibody
      • RAD17 homolog (S. pombe) antibody
      • RAD17 homolog antibody
      • Rad17 like protein antibody
      • RAD17, S. pombe, homolog of antibody
      • RAD17_HUMAN antibody
      • RAD17Sp antibody
      • Rad24 antibody
      • Rad24, mouse, homolog of antibody
      • Rad24, S. cerevisiae, homolog of antibody
      • RF C activator 1 homolog antibody
      • RF C/activator 1 homolog antibody
      • RF-C/activator 1 homolog antibody
      see all

    Anti-Rad17 antibody - Carboxyterminal end images

    • Ab50526, at 10ug/ml, staining Rad17 in methanol-acetone fixed HEK-293-T cells by immunofluorescence.

    References for Anti-Rad17 antibody - Carboxyterminal end (ab50526)

    ab50526 has not yet been referenced specifically in any publications.

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