Overview

  • Product nameAnti-Rad17 (phospho S645) antibody
    See all Rad17 primary antibodies
  • Description
    Rabbit polyclonal to Rad17 (phospho S645)
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, African Green Monkey, Orangutan
  • Immunogen

    Synthetic phospho peptide (Human) - which represents a portion of human Rad 17around serine 645.

  • Positive control
    • HeLa whole cell lysate treated with 10 Gy IR.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies that were not phospho-specific were removedby solid phase absorption. Antibodies specific for Rad 17pSer 645 were affinity purified using the phosphopeptideimmobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 85-88 kDa (predicted molecular weight: 77 kDa).
ICC/IF Use at an assay dependent dilution. PubMed: 20368419

Target

  • FunctionEssential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
  • Tissue specificityOverexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
  • Sequence similaritiesBelongs to the rad17/RAD24 family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
  • Cellular localizationNucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCYC antibody
    • Cell cycle checkpoint protein antibody
    • Cell cycle checkpoint protein RAD17 antibody
    • FLJ41520 antibody
    • HRAD 17 antibody
    • hRad17 antibody
    • R24L antibody
    • Rad 17 antibody
    • Rad 24 antibody
    • RAD1 (S. pombe) homolog antibody
    • RAD1 homolog antibody
    • RAD17 antibody
    • RAD17 homolog (S. pombe) antibody
    • RAD17 homolog antibody
    • Rad17 like protein antibody
    • RAD17, S. pombe, homolog of antibody
    • RAD17_HUMAN antibody
    • RAD17Sp antibody
    • Rad24 antibody
    • Rad24, mouse, homolog of antibody
    • Rad24, S. cerevisiae, homolog of antibody
    • RF C activator 1 homolog antibody
    • RF C/activator 1 homolog antibody
    • RF-C/activator 1 homolog antibody
    see all

Anti-Rad17 (phospho S645) antibody images



  • Predicted band size : 77 kDa


    Sample: Whole Cell Lysate (50µg/lane) from Hela cells treated with 10Gy IR or untreated.

    Rabbit anti-Rad 17 pSer645 (ab3620) used at 1:1,000 dilution

References for Anti-Rad17 (phospho S645) antibody (ab3620)

This product has been referenced in:
  • Shin MH  et al. ATM-dependent phosphorylation of the checkpoint clamp regulates repair pathways and maintains genomic stability. Cell Cycle 11:1796-803 (2012). Read more (PubMed: 22453082) »
  • Kumar A  et al. Nuclear phosphoinositide 3-kinase beta controls double-strand break DNA repair. Proc Natl Acad Sci U S A 107:7491-6 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20368419) »

See all 3 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More

I am sorry to hear that the results does not improve. I can offer you another antibody we have following antibodies against the same target

ab63442; http://www.abcam.com/Rad17-phospho-S645-antibody-ab63442.html

ab115876; http://www...

Read More

Well that's true. BSA reduces the background only, it does not have any effect on ab specificity.

Anyway to proceed further in this case, I would like to ask if you are happy in trying a different lot or a different antibody. Alternatively I ...

Read More

Thank you very much for your email.

We use 5% BSA (Sigma) in TBST for blocking and for diluting secondary and primaries. Milk in not recommended for phospho specific antibodies because milk contains casein which is a phosphoprotein; This is w...

Read More

Thank you for your enquiry regarding ab3620 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

The protocol looks fine to me however I would like...

Read More

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.



In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the app...

Read More

To our knowledge, these three antibodies have not been used in parallel, which would be required to determine if there are truely differences in the apparant molecular weight of the protein recognized by each antibody. The differences in the reporte...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"