Anti-Rad17 (phospho S645) antibody (ab63442)

Overview

  • Product nameAnti-Rad17 (phospho S645) antibody
    See all Rad17 primary antibodies
  • Description
    Rabbit polyclonal to Rad17 (phospho S645)
  • Specificityab63442 detects Rad17 only when phosphorylated at serine 645.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Rad17 around the phosphorylation site of serine 645 (P-A-SP-Q-P).

  • Positive control
    • HeLa cell extracts, treated with UV (15mins).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).

Target

  • FunctionEssential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
  • Tissue specificityOverexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
  • Sequence similaritiesBelongs to the rad17/RAD24 family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
  • Cellular localizationNucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCYC antibody
    • Cell cycle checkpoint protein antibody
    • Cell cycle checkpoint protein RAD17 antibody
    • FLJ41520 antibody
    • HRAD 17 antibody
    • hRad17 antibody
    • R24L antibody
    • Rad 17 antibody
    • Rad 24 antibody
    • RAD1 (S. pombe) homolog antibody
    • RAD1 homolog antibody
    • RAD17 antibody
    • RAD17 homolog (S. pombe) antibody
    • RAD17 homolog antibody
    • Rad17 like protein antibody
    • RAD17, S. pombe, homolog of antibody
    • RAD17_HUMAN antibody
    • RAD17Sp antibody
    • Rad24 antibody
    • Rad24, mouse, homolog of antibody
    • Rad24, S. cerevisiae, homolog of antibody
    • RF C activator 1 homolog antibody
    • RF C/activator 1 homolog antibody
    • RF-C/activator 1 homolog antibody
    see all

Anti-Rad17 (phospho S645) antibody images

  • All lanes : Anti-Rad17 (phospho S645) antibody (ab63442) at 1/500 dilution

    Lane 1 : HeLa cell extracts (5-30µg total protein) treated with UV (15mins)
    Lane 2 : HeLa cell extracts (5-30µg total protein) treated with UV (15mins) with Immunising peptide at 10 µg


    Predicted band size : 77 kDa
    Observed band size : 77 kDa

References for Anti-Rad17 (phospho S645) antibody (ab63442)

ab63442 has not yet been referenced specifically in any publications.

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