Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779)

Overview

  • Product nameAnti-Rad17 (phospho S645) antibody [EP1519Y]
    See all Rad17 primary antibodies
  • Description
    Rabbit monoclonal [EP1519Y] to Rad17 (phospho S645)
  • Specificityab75779 only detects Rad17 phosphorylated on Serine 645.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17

  • Positive control
    • HeLa cell lysates either untreated or treated with Lambda Phosphatase.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionEssential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
    • Tissue specificityOverexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
    • Sequence similaritiesBelongs to the rad17/RAD24 family.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
    • Cellular localizationNucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCYC antibody
      • Cell cycle checkpoint protein antibody
      • Cell cycle checkpoint protein RAD17 antibody
      • FLJ41520 antibody
      • HRAD 17 antibody
      • hRad17 antibody
      • R24L antibody
      • Rad 17 antibody
      • Rad 24 antibody
      • RAD1 (S. pombe) homolog antibody
      • RAD1 homolog antibody
      • RAD17 antibody
      • RAD17 homolog (S. pombe) antibody
      • RAD17 homolog antibody
      • Rad17 like protein antibody
      • RAD17, S. pombe, homolog of antibody
      • RAD17_HUMAN antibody
      • RAD17Sp antibody
      • Rad24 antibody
      • Rad24, mouse, homolog of antibody
      • Rad24, S. cerevisiae, homolog of antibody
      • RF C activator 1 homolog antibody
      • RF C/activator 1 homolog antibody
      • RF-C/activator 1 homolog antibody
      see all

    Anti-Rad17 (phospho S645) antibody [EP1519Y] images

    • All lanes : Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779) at 1/5000 dilution

      Lane 1 : HeLa cell lysates, untreated
      Lane 2 : HeLa cell lysates, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 77 kDa
      Observed band size : 77 kDa

    References for Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779)

    ab75779 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"