Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)


  • Product nameAnti-Rad17 (phospho S656) antibody [EPR3145]
    See all Rad17 primary antibodies
  • Description
    Rabbit monoclonal [EPR3145] to Rad17 (phospho S656)
  • Specificityab76012 only detects Rad17 phosphoryated on Serine 656.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Serine 656 of human Rad17.

  • Positive control
    • HeLa cell lysate treated with Calyculin A. Human breast carcinoma. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 77 kDa.

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
    • Tissue specificityOverexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
    • Sequence similaritiesBelongs to the rad17/RAD24 family.
    • Post-translational
      Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
    • Cellular localizationNucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCYC antibody
      • Cell cycle checkpoint protein antibody
      • Cell cycle checkpoint protein RAD17 antibody
      • FLJ41520 antibody
      • HRAD 17 antibody
      • hRad17 antibody
      • R24L antibody
      • Rad 17 antibody
      • Rad 24 antibody
      • RAD1 (S. pombe) homolog antibody
      • RAD1 homolog antibody
      • RAD17 antibody
      • RAD17 homolog (S. pombe) antibody
      • RAD17 homolog antibody
      • Rad17 like protein antibody
      • RAD17, S. pombe, homolog of antibody
      • RAD17_HUMAN antibody
      • RAD17Sp antibody
      • Rad24 antibody
      • Rad24, mouse, homolog of antibody
      • Rad24, S. cerevisiae, homolog of antibody
      • RF C activator 1 homolog antibody
      • RF C/activator 1 homolog antibody
      • RF-C/activator 1 homolog antibody
      see all

    Anti-Rad17 (phospho S656) antibody [EPR3145] images

    • All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012) at 1/10000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 77 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • ab76012, at 1/100-1/250 dilution, staining Rad17 in human breast carcinoma by immunohistochemistry using paraffin-embedded tissue.
    • ab76012, at 1/100-1/250 dilution, staining Rad17 in HeLa cells by immunofluorescence.

    References for Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012)

    ab76012 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76012.
    Please use the links above to contact us or submit feedback about this product.