Overview

  • Product nameAnti-RAD18 antibody
    See all RAD18 primary antibodies
  • Description
    Rabbit polyclonal to RAD18
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Fusion protein (Human) from the C-terminus with a proprietary tag.

  • Positive control
    • A549 cells GM637 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab79763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionE3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RAD18 family.
    Contains 1 RING-type zinc finger.
    Contains 1 SAP domain.
    Contains 1 UBZ-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810024C04Rik antibody
    • DNA repair protein rad18 antibody
    • E3 ubiquitin-protein ligase RAD18 antibody
    • EC 6.3.2.- antibody
    • hHR 18 antibody
    • hHR18 antibody
    • hRAD 18 antibody
    • hRAD18 antibody
    • MGC156682 antibody
    • Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
    • Postreplication repair protein hRAD18p antibody
    • Postreplication repair protein RAD18 antibody
    • RAD 18 antibody
    • RAD18 antibody
    • RAD18 homolog (S. cerevisiae) antibody
    • RAD18 homolog antibody
    • RAD18 S. cerevisiae homolog antibody
    • RAD18 S. cerevisiae homolog of antibody
    • RAD18 transcript variant antibody
    • RAD18_HUMAN antibody
    • Rad18sc antibody
    • Radiation sensitivity protein 18 antibody
    • RING finger protein 73 antibody
    • RNF 73 antibody
    • RNF73 antibody
    • Structural maintenance of chromosomes protein 6 antibody
    • YCR066W antibody
    • YCR66W antibody
    see all

Anti-RAD18 antibody images

  • Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.
  • Anti-RAD18 antibody (ab79763) at 1/1000 dilution + A549 crude extract

    Predicted band size : 56 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
    The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.

References for Anti-RAD18 antibody (ab79763)

This product has been referenced in:
  • Peng M  et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB ; Human . Read more (PubMed: 24966277) »
  • Zeman MK  et al. DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis. J Cell Biol 206:183-97 (2014). Human . Read more (PubMed: 25023518) »

See all 4 Publications for this product

Product Wall

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