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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
1/100 - 1/250.
Function E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Pathway Protein modification; protein ubiquitination.
Sequence similarities Belongs to the RAD18 family. Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.
Cellular localization Nucleus.
Information by UniProt
DNA repair protein rad18 antibody
E3 ubiquitin-protein ligase RAD18 antibody
Anti-RAD18 antibody images
Western blot - RAD18 antibody (ab97670)
Anti-RAD18 antibody (ab97670) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg Predicted band size : 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAD18 antibody (ab97670)
ab97670 at 1/100 dilution staining RAD18 in paraffin-embedded Cal27 xenograft tissue by immunohistochemistry.
References for Anti-RAD18 antibody (ab97670)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"