Overview

  • Product name
  • Description
    Rabbit polyclonal to RAD18
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids within 175-225 of Human RAD18 (NP_064550.2).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the RAD18 family.
      Contains 1 RING-type zinc finger.
      Contains 1 SAP domain.
      Contains 1 UBZ-type zinc finger.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810024C04Rik antibody
      • DNA repair protein rad18 antibody
      • E3 ubiquitin-protein ligase RAD18 antibody
      • EC 6.3.2.- antibody
      • hHR 18 antibody
      • hHR18 antibody
      • hRAD 18 antibody
      • hRAD18 antibody
      • MGC156682 antibody
      • Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
      • Postreplication repair protein hRAD18p antibody
      • Postreplication repair protein RAD18 antibody
      • RAD 18 antibody
      • RAD18 antibody
      • RAD18 homolog (S. cerevisiae) antibody
      • RAD18 homolog antibody
      • RAD18 S. cerevisiae homolog antibody
      • RAD18 S. cerevisiae homolog of antibody
      • RAD18 transcript variant antibody
      • RAD18_HUMAN antibody
      • Rad18sc antibody
      • Radiation sensitivity protein 18 antibody
      • RING finger protein 73 antibody
      • RNF 73 antibody
      • RNF73 antibody
      • Structural maintenance of chromosomes protein 6 antibody
      • YCR066W antibody
      • YCR66W antibody
      see all

    Images

    • ab99334 at 3µg/ml detecting RAD18 in HeLa whole cell lysate by IP.
      Lane 1: ab99334 IP at 3µg/mg of lysate
      Lane 2: IP with an antibody which recognizes an downstream epitope of RAD18
      Lane 3: IP control IgG
      In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
      Subsequent WB detection was performed using an antibody that recognises a downstream epitope of RAD18.
      Detection: Chemiluminescence with an exposure time of 3 seconds. Two bands represent, but one or both likely represent a post-translationally modified form.

    References

    ab99334 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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