Recombinant
RabMAb

Anti-RAD18 antibody [EPR8942] (ab157463)

Overview

  • Product name
    Anti-RAD18 antibody [EPR8942]
    See all RAD18 primary antibodies
  • Description
    Rabbit monoclonal [EPR8942] to RAD18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human RAD18 (UniProt Q9NS91).

  • Positive control
    • 293T, HCT-116, K562 and HeLa cell lysates; Permeabilized K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the RAD18 family.
      Contains 1 RING-type zinc finger.
      Contains 1 SAP domain.
      Contains 1 UBZ-type zinc finger.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810024C04Rik antibody
      • DNA repair protein rad18 antibody
      • E3 ubiquitin-protein ligase RAD18 antibody
      • EC 6.3.2.- antibody
      • hHR 18 antibody
      • hHR18 antibody
      • hRAD 18 antibody
      • hRAD18 antibody
      • MGC156682 antibody
      • Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
      • Postreplication repair protein hRAD18p antibody
      • Postreplication repair protein RAD18 antibody
      • RAD 18 antibody
      • RAD18 antibody
      • RAD18 homolog (S. cerevisiae) antibody
      • RAD18 homolog antibody
      • RAD18 S. cerevisiae homolog antibody
      • RAD18 S. cerevisiae homolog of antibody
      • RAD18 transcript variant antibody
      • RAD18_HUMAN antibody
      • Rad18sc antibody
      • Radiation sensitivity protein 18 antibody
      • RING finger protein 73 antibody
      • RNF 73 antibody
      • RNF73 antibody
      • Structural maintenance of chromosomes protein 6 antibody
      • YCR066W antibody
      • YCR66W antibody
      see all

    Images

    • All lanes : Anti-RAD18 antibody [EPR8942] (ab157463) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HCT-116 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa

    • Flow cytometric analysis of permeabilized K562 cells labeling RAD18 with ab157463 at 1/10 dilution (red) compared to a rabbit IgG negative control antibody (green).

    References

    ab157463 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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