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Rabbit monoclonal [EPR8942] to RAD18
Flow Cyt more details Unsuitable for:
ICC,IHC-P or IP
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human RAD18 (UniProt Q9NS91).
293T, HCT-116, K562 and HeLa cell lysates; Permeabilized K562 cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC,IHC-P or IP.
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Protein modification; protein ubiquitination.
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.
Information by UniProt
DNA repair protein rad18 antibody
E3 ubiquitin-protein ligase RAD18 antibody
Western blot - Anti-RAD18 antibody [EPR8942] (ab157463)
All lanes : Anti-RAD18 antibody [EPR8942] (ab157463) at 1/10000 dilution Lane 1 : 293T cell lysate Lane 2 : HCT-116 cell lysate Lane 3 : K562 cell lysate Lane 4 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 56 kDa
Flow Cytometry - Anti-RAD18 antibody [EPR8942] (ab157463)
Flow cytometric analysis of permeabilized K562 cells labeling RAD18 with ab157463 at 1/10 dilution (red) compared to a rabbit IgG negative control antibody (green).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"