RAD18 overexpression 293T lysate (whole cell) (ab94312)

Overview

  • Product name
    RAD18 overexpression 293T lysate (whole cell)
  • Description
    RAD18 overexpression lysate
  • General notes
    ab94312 is a 293T cell transfected lysate in which Human RAD18 has been transiently over-expressed using a pCMV-RAD18 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the RAD18 family. Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94312 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-RAD18 antibody (ab57447) at 1/500 dilution

    Lane 1 : RAD18 overexpression 293T lysate (whole cell) (ab94312)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94312 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab94312.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up