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Anti-Rad21 antibody - ChIP Grade (ab992)

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Overview

Product name

Anti-Rad21 antibody - ChIP Grade
See all Rad21 products (5) ...

Description

Rabbit polyclonal to Rad21 - ChIP Grade

Tested applications

ChIP, ICC/IF, WB, IPmore details

Cross reactivity

Reacts with

Mouse, Human, Xenopus laevis, Indian Muntjac

Immunogen

Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).

Positive control

HeLa cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Rad21 antibody - ChIP Grade (ab992)Western blot - Rad21 antibody - ChIP Grade (ab992) image (enlarge)

  • Western blot - Rad21 antibody (ab992)Western blot - Rad21 antibody (ab992) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

Sequence similarities

Belongs to the rad21 family.

Domain

The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.

Post-translational
modifications

Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK.

Cellular localization

Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis.

Target information above from: UniProt accessionO60216 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Double strand break repair protein rad21 homolog antibody
  • Double-strand-break repair protein rad21 homolog antibody
  • FLJ25655 antibody
  • FLJ40596 antibody
  • hHR21 antibody
  • HR21 antibody
  • HRAD21 antibody
  • KIAA0078 antibody
  • MCD1 antibody
  • Nuclear Matrix Protein 1 antibody
  • NXP 1 antibody
  • NXP-1 antibody
  • NXP1 antibody
  • Rad 21 antibody
  • RAD21 antibody
  • RAD21 homolog antibody
  • RAD21_HUMAN antibody
  • SCC1 antibody
  • SCC1 homolog antibody
see all

Database links

Anti-Rad21 antibody - ChIP Grade images:

  Western blot - Rad21 antibody - ChIP Grade (ab992)

Western blot - Rad21 antibody - ChIP Grade (ab992)

Anti-Rad21 antibody - ChIP Grade (ab992) at 1/1000 dilution

Predicted band size : 72 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 130 kDa (possible cross reactivity).

  Western blot - Rad21 antibody (ab992)

Western blot - Rad21 antibody (ab992)

Anti-Rad21 antibody - ChIP Grade (ab992) at 1/2000 dilution + HeLa whole cell extract. Extraction was achieved using RIPA buffer and 1% SDS. 25 µg of total protein was loaded

Secondary
Goat anti rabbit IgG (HRP) at 1/1500 dilution

Predicted band size : 72 kDa


A rad21 knock down negative control was employed.

This image is courtesy of an Abreview submitted on 9 September 2005. We do not have any further information relating to this image.

See Abreview

  Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)

Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)

ab992 at a 1/200 dilution staining Asynchronous and paraformaldehyde-fixed (4%) HeLa cells by immunocytochemistry. The antibody was incubated with the cells 30 minutes and then detected using a Cy3 conjugated Goat Anti-Mouse IgG (H+L) antibody.

This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.

See Abreview

  Western blot - Anti-Rad21 antibody - ChIP Grade (ab992)

Western blot - Anti-Rad21 antibody - ChIP Grade (ab992)

All lanes : Anti-Rad21 antibody - ChIP Grade (ab992) at 2 µg/ml

Lane 1 : HeLa at 50 µg
Lane 2 : HeLa at 15 µg
Lane 3 : HeLa at 5 µg
Lane 4 : 293T at 50 µg
Lane 5 : NIH3T3 at 50 µg


Predicted band size : 72 kDa


Exposure time : 30 seconds

References for Anti-Rad21 antibody - ChIP Grade (ab992)

This product has been referenced in:

  • Xiao Tet al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB; Human.Read more (PubMed: 21444719) »
  • Chatterjee Set al. Dynamic changes in binding of immunoglobulin heavy chain 3' regulatory region to protein factors during class switching. J Biol Chem 286:29303-12 (2011). ChIP; Mouse.Read more (PubMed: 21685395) »

See all 18 publications for this product

Publishing research using ab992? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"