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Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
Our Abpromise guarantee covers the use of ab992 in the following tested applications.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.
Used at a dilution of 1/200 for 30 min incubation (see Abreview for further information).
|WB||1/500 - 1/5000. Predicted molecular weight: 72 kDa.
Band observed at ~130 kDa.
|IP||Use a concentration of 1 - 4 µg/ml.|
ab992 at a 1/200 dilution staining Asynchronous and paraformaldehyde-fixed (4%) HeLa cells by immunocytochemistry. The antibody was incubated with the cells 30 minutes and then detected using a Cy3 conjugated Goat Anti-Mouse IgG (H+L) antibody.
This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.