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Western blot - Rad21 antibody - ChIP Grade (ab992)
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Western blot - Rad21 antibody (ab992)
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Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)
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Product Name
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Rad21 antibody - ChIP Grade
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See all Rad21 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rad21 - ChIP Grade
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Immunogen
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Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
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Reacts with
(species key)
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Hu, Ms, InMtj, Xl
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Specificity
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This antibody detects a band at 130kDa corresponding to Rad 21 and also a band of unknown origin at 75kDa.
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Tested applications
(see key)
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ChIP, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
ICC/IF: Used at a dilution of 1/200 for 30 min incubation (see Abreview for further information).
IP: Use at a concentration of 1 - 4 µg/ml.
WB: 1/500 - 1/5000. Predicted molecular weight: 72 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Chromatin and Nuclear Signaling >> Chromosome Structure >> Cohesins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
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Relevance
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The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad21 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rad21 antibody - ChIP Grade images, references, products related to ab992 and other tools.
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Rad21 antibody - ChIP Grade images:
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 Western blot - Rad21 antibody (ab992)
Rad21 antibody - ChIP Grade (ab992) at 1/2000 dilution + HeLa whole cell extract. Extraction was achieved using RIPA buffer and 1% SDS. 25 µg of total protein was loaded
Secondary
Goat anti rabbit IgG (HRP) at 1/1500 dilution
Predicted band size : 72 kDa
A rad21 knock down negative control was employed.
This image is courtesy of an Abreview submitted on 9 September 2005. We do not have any further information relating to this image.
See Abreview
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Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)
ab992 at a 1/200 dilution staining Asynchronous and paraformaldehyde-fixed (4%) HeLa cells by immunocytochemistry. The antibody was incubated with the cells 30 minutes and then detected using a Cy3 conjugated Goat Anti-Mouse IgG (H+L) antibody. This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.
See Abreview
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References for Rad21 antibody - ChIP Grade (ab992)
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This product has been used in: (two most recent references)
Degner SC et al. Cutting edge: developmental stage-specific recruitment of cohesin to CTCF sites throughout immunoglobulin loci during B lymphocyte development. J Immunol 182:44-8 (2009). ChIP; Mouse. PubMed: 19109133
Hadjur S et al. Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus. Nature 460:410-3 (2009). ChIP; Mouse. PubMed: 19458616
See all 12 publication references for this product.
If you publish research using ab992 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rad21
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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