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Western blot - Rad21 antibody - ChIP Grade (ab992)
Western blot - Rad21 antibody - ChIP Grade (ab992)
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Western blot - Rad21 antibody (ab992)
Western blot - Rad21 antibody (ab992)
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Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)
Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)
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Product Name 

Rad21 antibody - ChIP Grade

 

See all Rad21 antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Rad21 - ChIP Grade

Immunogen 

Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).

Reacts with 
(species key)

Hu, Ms, InMtj, Xl

Specificity 

This antibody detects a band at 130kDa corresponding to Rad 21 and also a band of unknown origin at 75kDa.

Tested applications 
(see key)

ChIP, ICC/IF, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ChIP

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• ICC/IF

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• WB

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


ChIP
(read Abreview)


ICC/IF
(read Abreview)


WB
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
ICC/IF: Used at a dilution of 1/200 for 30 min incubation (see Abreview for further information).
IP: Use at a concentration of 1 - 4 µg/ml.

WB: 1/500 - 1/5000. Predicted molecular weight: 72 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Chromatid Cohesion
Chromatin and Nuclear Signaling >> Chromosome Structure >> Cohesins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion

Relevance 

The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase.
RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells.

 

Alternative names Rad21 antibody - ChIP Grade (ab992)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

5885    (Human)
19357    (Mouse)
399129    (Xenopus laevis)

Omim

    

606462    (Human)

SwissProt

    

O60216    (Human)
Q61550    (Mouse)
O93310    (Xenopus laevis)

Unigene

    

81848    (Human)
182628    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rad21 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance!


See below for Rad21 antibody - ChIP Grade images, references, products related to ab992 and other tools.

 

Rad21 antibody - ChIP Grade images:

Product image  Western blot - Rad21 antibody - ChIP Grade (ab992)

Western blot - Rad21 antibody - ChIP Grade (ab992)

Rad21 antibody - ChIP Grade (ab992) at 1/1000 dilution

Predicted band size : 72 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Additional bands at : 130 kDa (possible cross reactivity).

 

Product image  Western blot - Rad21 antibody (ab992)

Western blot - Rad21 antibody (ab992)

Rad21 antibody - ChIP Grade (ab992) at 1/2000 dilution + HeLa whole cell extract. Extraction was achieved using RIPA buffer and 1% SDS. 25 µg of total protein was loaded

Secondary
Goat anti rabbit IgG (HRP) at 1/1500 dilution

Predicted band size : 72 kDa


A rad21 knock down negative control was employed.

This image is courtesy of an Abreview submitted on 9 September 2005. We do not have any further information relating to this image.

See Abreview

 

Product image  Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)

Immunocytochemistry/ Immunofluorescence - Rad21 antibody (ab992)

ab992 at a 1/200 dilution staining Asynchronous and paraformaldehyde-fixed (4%) HeLa cells by immunocytochemistry. The antibody was incubated with the cells 30 minutes and then detected using a Cy3 conjugated Goat Anti-Mouse IgG (H+L) antibody.

This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.

See Abreview

 

References for Rad21 antibody - ChIP Grade (ab992)

Specific publication references   This product has been used in: (two most recent references)

Degner SC et al. Cutting edge: developmental stage-specific recruitment of cohesin to CTCF sites throughout immunoglobulin loci during B lymphocyte development. J Immunol 182:44-8 (2009). ChIP; Mouse. PubMed: 19109133

Hadjur S et al. Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus. Nature 460:410-3 (2009). ChIP; Mouse. PubMed: 19458616


See all 12 publication references for this product.


If you publish research using ab992 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Rad21

 

Rad21 antibody - ChIP Grade - more information

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Rad21 antibody - ChIP Grade - related products:

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