Overview

  • Product name
    Anti-Rad22 antibody
  • Description
    Rabbit polyclonal to Rad22
  • Tested applications
    Suitable for: ELISA, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Purified recombinant Rad22 protein (Schizosaccharomyces pombe)

  • Positive control
    • Schizosaccharomyces pombe whole cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Whole serum
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S.cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating the strand annealing reaction between homologous DNA strands.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • DNA repair and recombination protein rad22 antibody
      • DNA repair protein Rad22 antibody
      • Rad 22 antibody

    Images

    • Anti-Rad22 antibody (ab63800) + S.pombe whole cell extract

      Predicted band size : 52 kDa
    • All lanes : Anti-Rad22 antibody (ab63800) at 1/2000 dilution (unpurified)

      Lane 1 : Molecular weight markers
      Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
      Lane 3 : Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain


      Predicted band size : 52 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

      ab63800 was used unpurified.

    References

    ab63800 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab63800.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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