Fusion protein containing the complete coding region (amino acids 1-425) of RAD50 expressed in E.coli.
Our Abpromise guarantee covers the use of ab89 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|ICC/IF||1/200. See Abreview.|
|WB||1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).|
|IP||Use at an assay dependent concentration.|
This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.
The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).
ab89 staining Rad50 in HPV transfected Hela cells by Immunocytochemistry/ Immunofluorescence. Cells were washed twice with phosphate-buffered saline (PBS), permeabilized with 0.5% Triton X-100 in CSK buffer (10 mM Hepes-KOH, pH 7.4; 300 mM sucrose; 100 mM NaCl; 3 mM KCl) for 2 min on ice, and fixed with 4% paraformaldehyde. Fixed cells were treated with 0.5% Triton X-100 in PBS, followed by 3 PBS washes for 5 min each at RT. After blocking with 3% bovine serum albumin (BSA) in PBS at RT for 30 min, the cells were incubated with primary antibody in antibody-binding solution (3% BSA in PBS) at RT for 30 min. An Alexa Fluor® 488 conjugated anti mouse secondary was used.