Anti-Rad50 antibody [20B5] (ab119708)


  • Product nameAnti-Rad50 antibody [20B5]
    See all Rad50 primary antibodies
  • Description
    Mouse monoclonal [20B5] to Rad50
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant protein of Human Rad50 purified from E.coli with His tag.

  • Positive control
    • HeLa, SK-N-MC, A431, HepG2, SK-BR3, Ramos, H9C2 and L929 cell lysates.



Our Abpromise guarantee covers the use of ab119708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 154 kDa.
IP Use at an assay dependent dilution. Use 5µl


  • FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Tissue specificityExpressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • Involvement in diseaseDefects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • Sequence similaritiesBelongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • DomainThe zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

Anti-Rad50 antibody [20B5] images

  • All lanes : Anti-Rad50 antibody [20B5] (ab119708) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SK-N-MC cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : SK-BR3 cell lysate
    Lane 6 : Ramos cell lysate
    Lane 7 : H9C2 cell lysate
    Lane 8 : L929 cell lysate

    Predicted band size : 154 kDa

References for Anti-Rad50 antibody [20B5] (ab119708)

ab119708 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Submitted Feb 18 2015