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Synthetic peptide (Human) - which represented a portion of human Rad 50 encoded in partby exons 2 and 3.
Our Abpromise guarantee covers the use of ab3622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 157 kDa (predicted molecular weight: 150 kDa).|
|IP||Use a concentration of 1 - 4 µg/ml.|
1. IP from 1mg extract with 1.25g Rabbit anti-Rad 50 (exon 2-3) (ab3622)
2. IP from 1mg extract with 1.25
Sample: Nuclear extract from Hela cells
for Western blot, affinity purified Rabbit anti-Rad 50 (ab3622) used at 1:4000 dilution