Overview

  • Product nameAnti-Rad50 antibody
    See all Rad50 primary antibodies
  • Description
    Rabbit polyclonal to Rad50
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Rad 50 encoded in partby exons 2 and 3.

  • Positive control
    • HeLa cell nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 157 kDa (predicted molecular weight: 150 kDa).
IP Use a concentration of 1 - 4 µg/ml.

Target

  • FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Tissue specificityExpressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
  • Involvement in diseaseDefects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
  • Sequence similaritiesBelongs to the SMC family. RAD50 subfamily.
    Contains 1 zinc-hook domain.
  • DomainThe zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA repair protein RAD50 antibody
    • hRAD50 antibody
    • hsRAD50 antibody
    • Mrell antibody
    • NBSLD antibody
    • Rad 50 antibody
    • RAD50 2 antibody
    • rad50 antibody
    • RAD50 homolog (S cerevisiae) antibody
    • RAD50 homolog antibody
    • RAD50 PEN antibody
    • RAD50, S. cerevisiae, homolog of antibody
    • RAD50_HUMAN antibody
    • RAD502 antibody
    • Rad50l antibody
    • Truncated RAD50 protein antibody
    see all

Anti-Rad50 antibody images

  • Lanes:

    1. IP from 1mg extract with 1.25g Rabbit anti-Rad 50 (exon 2-3) (ab3622)

    2. IP from 1mg extract with 1.25µg Rabbit anti-Rad 50 (exon 13-14) (ab3623)

    Sample: Nuclear extract from Hela cells

    for Western blot, affinity purified Rabbit anti-Rad 50 (ab3622) used at 1:4000 dilution

References for Anti-Rad50 antibody (ab3622)

This product has been referenced in:
  • Goldstein M  et al. Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair. Proc Natl Acad Sci U S A 110:16874-9 (2013). IP, ChIP . Read more (PubMed: 24082117) »
  • Tommiska J  et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer 118:2911-6 (2006). Read more (PubMed: 16385572) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Zebrafish Cell lysate - whole cell (whole embryo 2dpf and 3dpf)
Specification whole embryo 2dpf and 3dpf
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Jude Rosenthal

Verified customer

Submitted Aug 29 2014

Thank you for your enquiry. I have been in contact with the originator of this antibody and this antibody will probably not work with Xenopus rad50. Exact sequence information is proprietary. I hope this information helps, please do not hesitate t...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab3622. We have not had any other reports of problems with this antibody and since multiple vials have worked for you in the past, my assumption is that the late...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"