Recombinant Anti-Rad51 antibody [14B4] (ab213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [14B4] to Rad51
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Rad51 antibody [14B4]
See all Rad51 primary antibodies -
Description
Mouse monoclonal [14B4] to Rad51 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human Rad51 aa 1-338.
Database link: Q06609 -
Positive control
- WB: RAD51 shRNA transfected (+) 293T whole cell extracts, Jurkat, Raji and NCI-H929 whole cell lysate. IHC-P: BT483 xenoograft tissue, human colon esophagus tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The production method has changed from hybridoma to recombinant on 22nd March 2024
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
14B4 -
Myeloma
NS1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/100 - 1/1000.
|
WB | (5) |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
Please see WB protocol in image legend. |
ICC/IF | (2) |
1/100 - 1/1000.
|
Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. Please see WB protocol in image legend. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
Tissue specificity
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
Involvement in disease
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
Domain
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
Post-translational
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 5888 Human
- Entrez Gene: 19361 Mouse
- Entrez Gene: 499870 Rat
- Omim: 179617 Human
- SwissProt: Q06609 Human
- SwissProt: Q08297 Mouse
- Unigene: 631709 Human
- Unigene: 330492 Mouse
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Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Images
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All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa10% SDS-PAGE
Running condition: 80V, 15min; 140V, 40 min
Transfer condition: Semi-dry, 18 V, 60 min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60 min.
Primary antibody incubation: 4°C overnight.
Washing condition: 5 ml TBST, 4 x 5 min.
Chemiluminescent HRP Substrate.
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
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All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution
Lane 1 : Non-transfected 293T (-) whole cell extracts
Lane 2 : RAD51 shRNA transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody
Developed using the ECL technique.
Predicted band size: 37 kDa10% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
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Immunohistochemical analysis of paraffin-embedded BT483 xenoograft tissue labeling RAD51 with ab213 at 1/200 dilution. Nuclear staining is observed. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min.
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Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg
Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 42,45 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed Mock and treated HeLa cells labelling Rad51 with ab213 at 1/500 dilution (Green). Blue: Fluoroshield with DAPI
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ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (83)
ab213 has been referenced in 83 publications.
- Cahuzac M et al. Pre-activation of autophagy impacts response to olaparib in prostate cancer cells. Commun Biol 5:251 (2022). PubMed: 35318456
- Chakraborty S et al. Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks. iScience 25:104142 (2022). PubMed: 35434547
- Guh CY et al. XPF activates break-induced telomere synthesis. Nat Commun 13:5781 (2022). PubMed: 36184605
- Adolph MB et al. RADX controls RAD51 filament dynamics to regulate replication fork stability. Mol Cell 81:1074-1083.e5 (2021). PubMed: 33453169
- Maiani E et al. AMBRA1 regulates cyclin D to guard S-phase entry and genomic integrity. Nature 592:799-803 (2021). PubMed: 33854232