Overview

  • Product name
    Anti-Rad51 antibody [14B4]
    See all Rad51 primary antibodies
  • Description
    Mouse monoclonal [14B4] to Rad51
  • Specificity
    Specific for human Rad51.
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-138 of Human Rad51

  • Positive control
    • T24 Bladder carcinoma cells and recombinant fusion protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    14B4
  • Myeloma
    NS1
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
    • Tissue specificity
      Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
    • Involvement in disease
      Breast cancer
      Mirror movements 2
      Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
    • Sequence similarities
      Belongs to the RecA family. RAD51 subfamily.
      Contains 1 HhH domain.
    • Domain
      The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
    • Post-translational
      modifications
      Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
      Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRCA1/BRCA2 containing complex, subunit 5 antibody
      • BRCC 5 antibody
      • BRCC5 antibody
      • DNA repair protein RAD51 homolog 1 antibody
      • DNA repair protein rhp51 antibody
      • FANCR antibody
      • hRAD51 antibody
      • HsRAD51 antibody
      • HsT16930 antibody
      • MRMV2 antibody
      • Rad 51 antibody
      • RAD51 antibody
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
      • RAD51 homolog A antibody
      • RAD51 homolog antibody
      • RAD51 recombinase antibody
      • RAD51, S. cerevisiae, homolog of antibody
      • RAD51_HUMAN antibody
      • RAD51A antibody
      • RECA antibody
      • RecA like protein antibody
      • RecA, E. coli, homolog of antibody
      • Recombination protein A antibody
      see all

    Anti-Rad51 antibody [14B4] images

    • Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + Jurkat whole cell extract at 30 µg
      Developed using the ECL technique

      Predicted band size : 37 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of BT483 xenograft labeling RAD51 with ab213 at 1/200.

    • ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg

      Secondary
      HRP conjugated anti-mouse IgG at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 37 kDa
      Observed band size : 42,45 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

    References for Anti-Rad51 antibody [14B4] (ab213)

    This product has been referenced in:
    • Rasmussen RD  et al. Enhanced efficacy of combined HDAC and PARP targeting in glioblastoma. Mol Oncol N/A:N/A (2016). WB ; Human . Read more (PubMed: 26794465) »
    • Dooley KE  et al. Tandemly Integrated HPV16 Can Form a Brd4-Dependent Super-Enhancer-Like Element That Drives Transcription of Viral Oncogenes. MBio 7:N/A (2016). Read more (PubMed: 27624132) »

    See all 51 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 15 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Sample
    Human Cell (293T)
    Specification
    293T
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 13 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Sample
    Human Tissue sections (Lovo Xenograft)
    Specification
    Lovo Xenograft
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 17 2013

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

    (1) R...

    Read More

    Thank you for your email.

    I would be happy to process refunds for ab213 and ab68715. Could you however let me know why the customer expecting a 170kDa band for ab58101 which should be nearly 130-140 kDa.

    Thanks!

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and have following further questions for better understanding of the problem;

    -...

    Read More

    Thank you for contacting Abcam.

    I just had a few more protocol questions for you regarding the problems you are having with ab213 in western blot.

    1 - Why was 2 different lysis buffers used in the extraction protocol? Either of them...

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    I am happy to help. Best of luck in your experiments!

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab63801.

    To check the status of the order please contact ou...

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    Thank you for contacting us. I am sorry to hear that the antibody is giving problems. As we discussed yesterday over the phone, the following suggestions might help to get rid of the red blob you are seeing in the nuclei.: 1) use less antibody (...

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