Overview

  • Product nameAnti-Rad51 antibody
    See all Rad51 primary antibodies
  • Description
    Chicken polyclonal to Rad51
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant full length protein (Human)

  • Positive control
    • HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab63802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/7000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in diseaseBreast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 antibody images

  • All lanes : Anti-Rad51 antibody (ab63802) at 1/2000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Hela cell extract


    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells, staining Rad51 with ab63802.

    Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 in 1X PBS and blocked with 5% BSA for 15 mins at 20°C. Samples were incubated with primary antibody (1/500 in 5% BSA in PBS) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-chicken polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-Rad51 antibody (ab63802)

ab63802 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell line)
Loading amount 40 µg
Specification HeLa cell line
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Oct 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Specification MEF
Fixative Formaldehyde
Permeabilization Yes - 0.5% Tritón X+PBS1X
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line)
Specification HeLa cell line
Fixative Formaldehyde
Permeabilization Yes - 0.5% Tritón X+PBS1X
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"