Anti-Rad51 antibody - BSA and Azide free (ab88572)
Key features and details
- Mouse polyclonal to Rad51 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Rad51 antibody - BSA and Azide free
See all Rad51 primary antibodies -
Description
Mouse polyclonal to Rad51 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein within Human Rad51 aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: lysate from PC-12, Raw 264.7, Jurkat, NIH 3T3 cells or Human colon tissue. IHC: Human testis. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
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IHC-P | (1) |
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. The microwave method is recommended.
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ICC/IF | (4) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. The microwave method is recommended. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. -
Tissue specificity
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. -
Involvement in disease
Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers. -
Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain. -
Domain
The nuclear localization may reside in the C-terminus (between 259 and 339 AA). -
Post-translational
modificationsUbiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 5888 Human
- Entrez Gene: 19361 Mouse
- Entrez Gene: 499870 Rat
- Omim: 179617 Human
- SwissProt: Q06609 Human
- SwissProt: Q08297 Mouse
- Unigene: 631709 Human
- Unigene: 330492 Mouse
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Alternative names
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
see all
Images
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Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/ml + Human colon lysate at 50 µg
Predicted band size: 37 kDa -
Immunofluorescent staining of Rad51 in HeLa cells using ab88572 at 10 µg/ml.
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ab88572 at 3µg/ml staining Rad51 in formalin-fixed, paraffin-embedded Human testis tissue.
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Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/ml + Rat PC-12 cell lysate at 50 µg
Predicted band size: 37 kDa -
Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/ml + Raw 264.7 cell lysate (Mouse) at 50 µg
Predicted band size: 37 kDa -
Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/ml + Human Jurkat cell lysate at 50 µg
Predicted band size: 37 kDa -
Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/ml + NIH 3T3 cell lysate (Mouse) at 50 µg
Predicted band size: 37 kDa -
All lanes : Anti-Rad51 antibody - BSA and Azide free (ab88572) at 1 µg/ml
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 37 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (51)
ab88572 has been referenced in 51 publications.
- Wang W et al. Studying the mechanism of sperm DNA damage caused by folate deficiency. J Cell Mol Med 26:776-788 (2022). PubMed: 34953021
- Li WF et al. Production of Pyracantha Polysaccharide-Iron(III) Complex and Its Biologic Activity. Molecules 26:N/A (2021). PubMed: 33808420
- Wang D et al. ATM-phosphorylated SPOP contributes to 53BP1 exclusion from chromatin during DNA replication. Sci Adv 7:N/A (2021). PubMed: 34144977
- Zhang S et al. RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis. Sci Adv 7:eabg8205 (2021). PubMed: 34678064
- Zhang W et al. Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells. Aging Cell 20:e13307 (2021). PubMed: 33455051