Overview

  • Product name
  • Description
    Mouse polyclonal to Rad51
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human protein (NP_002866.2)

  • Positive control
    • WB: lysate from PC-12, Raw 264.7, Jurkat, NIH 3T3 cells or Human colon tissue. IHC: Human testis. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. The microwave method is recommended.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificity
    Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in disease
    Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • Sequence similarities
    Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • Domain
    The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • FANCR antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 antibody images

  • Anti-Rad51 antibody (ab88572) at 1 µg/ml + Human colon lysate at 50 µg

    Predicted band size : 37 kDa
  • Immunofluorescent staining of Rad51 in HeLa cells using ab88572 at 10 µg/ml.
  • ab88572 at 3µg/ml staining Rad51 in formalin-fixed, paraffin-embedded Human testis tissue.
  • Anti-Rad51 antibody (ab88572) at 1 µg/ml + PC-12 cell lysate at 50 µg

    Predicted band size : 37 kDa
  • Anti-Rad51 antibody (ab88572) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 37 kDa
  • Anti-Rad51 antibody (ab88572) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 37 kDa
  • Anti-Rad51 antibody (ab88572) at 1 µg/ml + NIH 3T3 cell ysate at 50 µg

    Predicted band size : 37 kDa
  • All lanes : Anti-Rad51 antibody (ab88572) at 1 µg/ml

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 37 kDa

References for Anti-Rad51 antibody (ab88572)

This product has been referenced in:
  • Cahyanti D  et al. Rad51 Expression in Nasopharyngeal Carcinoma and Its Association with Tumor Reduction: A Preliminary Study in Indonesia. Iran J Pathol 11:155-60 (2016). Read more (PubMed: 27499778) »
  • Arora A  et al. Clinicopathological and prognostic significance of RECQL5 helicase expression in breast cancers. Carcinogenesis 37:63-71 (2016). Read more (PubMed: 26586793) »

See all 11 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 22 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Esophageal tumor samples from clinic)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate antigen repair solution
Permeabilization
No
Specification
Esophageal tumor samples from clinic
Blocking step
Endogenous peroxidase blockers as blocking agent for 15 minute(s) · Concentration: 0% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 09 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (LLC-PK1 cell line)
Permeabilization
Yes - 0.5% Triton-X 100 in PBS
Specification
LLC-PK1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 02 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (EC109)
Permeabilization
Yes - 0.1% Triton X-100
Specification
EC109
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 22 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Esophageal Tumor Cells)
Gel Running Conditions
Non-reduced Denaturing (10% SDS)
Loading amount
80 µg
Specification
Esophageal Tumor Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Qingyuan Yang

Verified customer

Submitted Dec 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Brain tumour cell line T98)
Specification
Brain tumour cell line T98
Fixative
Paraformaldehyde
Permeabilization
Yes - 10% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Dr. Henry King

Verified customer

Submitted Mar 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount
50 µg
Specification
Mouse ES Cells
Gel Running Conditions
Reduced Denaturing (12% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 28 2012

Thank you for your new contact and clarifying the fixation method.

This message was sent to you previously. Would you be so kind to let me know how you wish to proceed.

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