This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Rad51 (phospho T309) antibody (ab31769)


  • Product nameAnti-Rad51 (phospho T309) antibody
    See all Rad51 primary antibodies
  • Description
    Rabbit polyclonal to Rad51 (phospho T309)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity

    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Rad51 aa 300 to the C-terminus (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab33130, ab33130, ab33130, ab33130)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ELISA Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab33130). Optimal dilutions/concentrations should be determined by the end user.


  • FunctionPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • Tissue specificityHighly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • Involvement in diseaseBreast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • Sequence similaritiesBelongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • DomainThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • Post-translational
    Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 (phospho T309) antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Elisa using ab31769 at varying antibody concentrations. Curve_SPL3 indicates binding to the Rad51 (phospho T309) peptide. Curve_SPL4 indicates <2% crossreactivity with the unmodified Rad51 control peptide.
  • All lanes : Anti-Rad51 (phospho T309) antibody (ab31769) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Rad51 (phospho T309) antibody (ab31769)

ab31769 has not yet been referenced specifically in any publications.

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