Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent dilution.
FunctionInvolved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Sequence similaritiesBelongs to the RAD52 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.